The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment.

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Immunocytochemical staining of HepG2 cells with Mannose Receptor (CD206) antibody . Nuclei were stained blue with DAPI; Mannose Receptor (CD206) was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Flow cytometric analysis of Mannose Receptor (CD206) expression in HepG2 cells using Mannose Receptor (CD206) antibody . Green, isotype control; red, Mannose Receptor (CD206).

Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using mannose receptor (CD206) antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using mannose receptor (CD206) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mannose receptor (CD206) antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using mannose receptor (CD206) antibody . Mannose receptor (CD206) expression in wild-type (WT) and MRC1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mannose receptor (CD206) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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