Nuclear factor kB (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel, p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

推荐稀释比 WB:1/1000-1/5000; IHC:P:1/1000-1/5000; ICC/IF:1/50-1/100; IP:1/20-1/50

Western blot analysis of NF-KB p65 in Hela lysates using NF-KB p65 antibody.

Western blot analysis of NF-KB p65 in K562, 3T3, Hela lysates using NF-KB p65 antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NF-KB p65 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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