Cell division cycle protein 27 homolog (CDC27) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

推荐稀释比 WB:1/500-1/1000;IHC-P:1/50-1/100;ICC/IF:1/50;FC:1/50-1/100

Western blot analysis of CDC27 in lysates of MCF-7 and BRL using CDC27 antibody.

Immunohistochemistry analysis of paraffin-embedded Human ovarian carcinoma using CDC27 antibody.High pressure and temperature Tris/EDTA (PH 9.0) used for antigen retrieval.The brown color represents the positive signal observed with CDC27 antibody.

Immunocytochemistry analysis of CDC27(green) in 293T cell line using CDC27 antibody,and DAPI(blue).

Flow Cytometry analysis of K562 cell line stained with CDC27 antibody(Red).Blue line histogram represents nonspecific negative control

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