Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.

推荐稀释比 WB:1/500-1/1000;IHC:1/50-1/100;IP:1/20

Western blot analysis of LC3A in 3T3, mouse heart, mouse brain lysates using LC3A antibody.

Western blot analysis of LC3A in K562, rat Brain, Hela lysates using LC3A antibody.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using LC3A antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.