Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.
稀释比 WB:1/500-1/1000; IHC:1/50-1/100;IF:1/50-1/200; FC:1/50-1/100
Western blot
Immunohistochemistry
Immunofluorescence
-25~-15℃保存
Ubiquitin Rabbit pAb 兔多克隆抗体
产品信息
|
产品名称 |
产品编号 |
规格 |
|
Ubiquitin Rabbit pAb 兔多克隆抗体 |
30007ES50 |
50 μL |
|
30007ES60 |
100 μL |
产品简介
该基因编码泛素，这是已知最保守的蛋白质之一。泛素在26S蛋白小体降解的细胞蛋白靶向中起着重要作用。它还参与染色质结构的维护、基因表达的调节和应激反应。泛素是由多泛素链或融合到不相关蛋白的单个泛素部分组成的前体蛋白合成的。该基因由泛素编码序列的3个直接重复序列组成，没有间隔序列。因此，在最后一次重复后，该蛋白表达为多泛素前体和最后一个氨基酸。这种蛋白质的一种异常形式已经在阿尔茨海默病和唐氏综合症患者中被检测到。IκB、p53、cdc25A 和 Bcl-2 等多种蛋白已被证实是泛素-蛋白酶体过程的靶标，可参与细胞周期进程、分化、细胞应激反应和凋亡的调节.
产品性质
|
别名 |
FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A; |
|
SwissProt ID |
P0CG47 |
|
基因ID |
7314 |
|
免疫原 |
A synthesized peptide derived from human Ubiquitin. |
|
缓冲液 |
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
|
克隆类型 |
Polyclonal Antibody |
|
亚型 |
IgG |
|
宿主 |
Rabbit |
|
物种反应 |
Human, Mouse, Rat |
|
推荐应用 |
WB,IHC-P,ICC/IF,FC |
|
纯化方式 |
Affinity Purified |
应用
稀释比 WB:1/500-1/1000; IHC:1/50-1/100;IF:1/50-1/200; FC:1/50-1/100
储存条件
-25~-15℃保存
注意事项
1. 避免反复冻融。
2. 本产品仅作科研用途。
3. 为了您的安全和健康，请穿实验服并佩戴一次性手套操作。
产品数据
Western blot
Western blot analysis of Ubiquitin in 293T lysates using Ubiquitin antibody.
Immunohistochemistry
Immunohistochemistry analysis of paraffin-embedded Human colon using Ubiquitin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunofluorescence
Immunofluorescence analysis of Ubiquitin in Raji using Ubiquitin antibody.
Ver.CN20240719
