Up-regulation of MELK by E2F1 promotes the proliferation in cervical cancer cells
Hongzhi Sun, Hongmei Ma, Hao Zhang, Minjun Ji
Journal：International Journal of Biological Sciences
IF：6.58
DOI：10.7150/ijbs.62517
PMID：34671205
Published：2021-09-07
research field：肿瘤学分子生物学胃肠病学微生物学基因调控
Abstract
Cervical cancer is a common gynecologic cancer and a frequent cause of death. In this study, we investigated the role of MELK (maternal embryonic leucine zipper kinase) in cervical cancer. We found that HPV 18 E6/E7 promoted MELK expression by activating E2F1. MELK knockdown blocked cancer cells growth. Furthermore, we used MELK-8A to inhibit the kinase activity of MELK and caused the G2/M phase arrest of cancer cells. Under the treatment of inhibitors, Hela cells formed multipolar spindles and eventually underwent apoptosis. We also found that MELK is involved in protein translation and folding during cell division through the MELK interactome and the temporal proteomic analysis under inhibition with MELK-8A. Altogether, these results suggest that MELK may play a vital role in cancer cell proliferation and indicate a potential therapeutic target for cervical cancer.
本文使用的Yeasen产品
