Protocol for the identification and expression analysis of a cytoplasmic membrane-localized protein STING
Jiaqi Shi, Caiqi Liu, Shengnan Luo, Yingying Zhang, Mingwei Li, Meng Zhou, Mingjiao Weng, Xiaobo Li, Tongsen Zheng
Journal：STAR Protocols
IF：0
DOI：10.1016/j.xpro.2023.102172
PMID：
Published：2023-03-20
research field：分子生物学细胞生物学免疫学
Abstract
Summary Here, we present a protocol for the detection of the two STING isoforms (erSTING and pmSTING) in human peripheral blood mononuclear cells or mouse splenocytes using Western blot and PCR. We detail steps to construct plasmids encoding each isoform and transfer them into mouse and human cell lines. Finally, we describe how to detect cell membrane localization of pmSTING using flow cytometry, immunoprecipitation, and immunofluorescence. This protocol is applicable for proteins with well-predicted topological structures. For complete details on the use and execution of this protocol, please refer to Li et al.1.
本文使用的Yeasen产品
