保幼激素类似物降低飞蝗脂肪酸结合蛋白表达影响脂质积累

Abstract

Simple SummaryJuvenile hormone analogues (JHAs) are widely used insect growth regulators that interfere with insect development and metamorphosis. However, the molecular mechanisms underlying their toxicity remain incompletely understood. In this study, we investigated the effects of two JHAs, pyriproxyfen and hydroprene, on hormonal regulation and lipid metabolism in the migratory locust (Locusta migratoria). Transcriptomic analyses revealed that JHA exposure disrupted the balance between juvenile hormone and ecdysteroid signaling pathways and significantly altered the expression of genes involved in molting and development. In addition, lipid metabolism-related pathways were strongly affected, particularly the fatty acid-binding protein geneMg-FABP, which showed marked downregulation after treatment. Structural and molecular docking analyses further demonstrated that LmMg-FABP possesses a conserved lipid-binding structure capable of interacting with multiple fatty acid ligands. These findings suggest that JHAs not only disrupt endocrine signaling but also impair lipid transport and metabolic homeostasis. This study provides new insight into the molecular mechanisms of JHA toxicity and identifies FABP-associated lipid metabolism as a potential target for the development of more selective insect growth regulators.Juvenile hormone (JH) analog insecticides are widely used in pest management because of their ability to disrupt insect growth and metamorphosis; however, the molecular mechanisms linking endocrine disruption to metabolic dysregulation remain incompletely understood. In addition to their established roles in diapause and developmental regulation, JH signaling pathways have also been implicated in carbohydrate and lipid metabolism. In the present study, we investigated the effects of two JH analogs, pyriproxyfen and hydroprene, on the migratory locust,Locusta migratoria, with particular emphasis on lipid metabolic regulation and the function of midgut-enric