暴露于帕金森环境毒素的人类神经元模型中的染色质可及性和转录组

Abstract

Parkinson’s disease (PD) is a complex neurodegenerative disorder where environmental factors play a predominant role in sporadic cases. While environmental toxins are implicated in PD pathogenesis via epigenetic pathways, the specific alterations in chromatin accessibility induced by these toxins remain poorly characterized. To address this gap, we established in vitro models using the human neuroblastoma cell line SH-SY5Y, a well-established neuronal model, exposed to the PD-associated environmental toxins rotenone and MPP⁺. We performed RNA sequencing (RNA-seq) to profile the transcriptome and Assay for Transposase-Accessible Chromatin sequencing (ATAC-seq) to map genome-wide chromatin accessibility landscapes under toxin-exposed conditions. This integrated dataset provides a comprehensive resource detailing both gene expression and chromatin accessibility dynamics in response to PD-relevant environmental neurotoxins and will facilitate investigations into transcriptional regulation, biomarker discovery, and the epigenetic basis of environmentally linked sporadic PD.