The protein encoded by this gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citrate from oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. This protein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HeLa cells using Citrate synthetase antibody , Top panel: wild-type (WT); Bottom panal: Citrate synthetase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Citrate synthetase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Citratesynthetase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Citratesynthetase antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Citrate synthetase expression in HepG2 cells using Citrate synthetase antibody . Green, isotype control; red, Citrate synthetase.

Immunocytochemical staining of HepG2 cells with Citrate synthetase antibody . Nuclei were stained blue with DAPI; Citrate synthetase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Citrate synthetase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Citrate synthetase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Citrate synthetase antibody . Citrate synthetase expression in wild type (WT) and Citrate synthetase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with Citrate synthetase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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