The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

推荐稀释比 WB: 1/2000-1/10000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Cyclin D1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cyclin D1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Cyclin D1 antibody , Top panel: wild-type (WT); Bottom panal: Cyclin D1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of Cyclin D1 expression in C2C12 cells using Cyclin D1 antibody . Green, isotype control; red, Cyclin D1.

Immunocytochemical staining of C2C12 cells with Cyclin D1 antibody . Nuclei were stained blue with DAPI; Cyclin D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Cyclin D1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin D1 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Cyclin D1 antibody . Cyclin D1 expression in wild type (WT) and Cyclin D1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Cyclin D1 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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