Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.

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Validation of Hexokinase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Hexokinase 1 antibody and analyzed using CytoFLEX.

Flow cytometric analysis of Hexokinase 1 expression in HT-1080 cells using Hexokinase 1 antibody . Green, isotype control; red, Hexokinase 1

Immunocytochemical staining of HeLa cells using Hexokinase 1 antibody , Top panel: wild-type (WT); Bottom panal: Hexokinase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Hexokinase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Immunocytochemical staining of HT-1080 cells with Hexokinase 1 antibody . Nuclei were stained blue with DAPI; Hexokinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Hexokinase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hexokinase 1 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Hexokinase 1 antibody . Hexokinase 1 expression in wild type (WT) and Hexokinase 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Hexokinase 1 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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