The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Validation of PKR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-PKR (T451) antibody and analyzed using CytoFLEX.

Flow cytometric analysis of phospho-PKR (T451) expression in HT-1080 cells using phospho-PKR (T451) antibody . Green, isotype control; red, phospho-PKR (T451).

Immunocytochemical staining of HT-1080 cells with Phospho-PKR (T451)  antibody . Nuclei were stained blue with DAPI; Phospho-PKR (T451)  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-PKR (T451) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PKR (T451) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phospho-PKR (T451) antibody . Phospho-PKR (T451) expression in wild type (WT) and phospho-PKR (T451) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospho-PKR (T451) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Store at -20℃ for one year.