This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants.

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Flow cytometric analysis of ACSS2 expression in HepG2 cells using ACSS2 antibody . Green, isotype control; red, ACSS2.

Western blotting analysis using ACSS2 antibody . Total cell lysates (45 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ACSS2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . ACSS2, acyl-CoA synthetase short chain family member 2.

Western blotting analysis using ACSS2 antibody . ACSS2 expression in wild type (WT) and ACSS2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ACSS2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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