This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Flow cytometric analysis of Acetyl-CoA acetyltransferase 1 expression in HepG2 cells using Acetyl-CoA acetyltransferase 1 antibody . Green, isotype control; red, Acetyl-CoA acetyltransferase 1.

Validation of Acetyl-CoA acetyltransferase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Acetyl-CoA acetyltransferase 1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HT-1080 cells with Acetyl-CoA acetyltransferase 1 antibody . Nuclei were stained blue with DAPI;Acetyl-CoA acetyltransferase 1 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using acetyl-CoA acetyltransferase 1 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

Western blotting analysis using Acetyl-CoA acetyltransferase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Acetyl-CoA acetyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Acetyl-CoA acetyltransferase 1 antibody . Acetyl-CoA acetyltransferase 1 expression in wild type (WT) and acetyl-CoA acetyltransferase 1 shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Acetyl-CoA acetyltransferase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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