The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.

推荐稀释比 WB: 1/1000-1/10000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Phospho-GSK3 beta (Ser9) expression in HepG2 cells using Phospho-GSK3 beta (Ser9) antibody . Green, isotype control; red, Phospho-GSK3 beta (Ser9).

Immunocytochemical staining of HepG2 cells with Phospho-GSK3 beta (Ser9) antibody . Nuclei were stained blue with DAPI; Phospho-GSK3 beta (Ser9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Phospho-GSK3 beta (Ser9) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-GSK3 beta (Ser9) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phospho-GSK3 beta (Ser9) antibody . Phospho-GSK3 beta (Ser9) expression in wild type (WT) and phospho-GSK3 beta (Ser9) shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-GSK3 beta (Ser9) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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