This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants.

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Immunocytochemical staining of HepG2 cells with PRKRA antibody . Nuclei were stained blue with DAPI; PRKRA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using PRKRA antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKRA antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using PRKRA antibody . PRKRA expression in wild type (WT) and PRKRA shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PRKRA antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of PRKRA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PRKRA antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using PRKRA antibody , Top panel: wild-type (WT); Bottom panal: PRKRA shRNA knockdown (KD). Nuclei were stained blue with DAPI; PRKRA was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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