ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

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Flow cytometric analysis of ATP citrate lyase expression in HepG2 cells using ATP citrate lyase antibody . Green, isotype control; red, ATP citrate lyase.

Immunocytochemical staining of HepG2 cells with ATP citrate lyase antibody . Nuclei were stained blue with DAPI; ATP citrate lyase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using ATP citrate lyase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP citrate lyase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using ATP citrate lyase antibody . ATP citrate lyase expression in wild type (WT) and ATP citrate lyase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ATP citrate lyase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using ATP citrate lyase antibody , Top panel: wild-type (WT); Bottom panal: ATP citrate lyase shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP citrate lyase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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