This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.

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Flow cytometric analysis of Nuclear mitotic apparatus protein 1 expression in HepG2 cells using Nuclear mitotic apparatus protein 1 antibody . Green, isotype control; red, Nuclear mitotic apparatus protein 1.

Immunocytochemical staining of HepG2 cells with Nuclear mitotic apparatus protein 1 antibody . Nuclei were stained blue with DAPI; Nuclear mitotic apparatus protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Nuclear mitotic apparatus protein 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nuclear mitotic apparatus protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using Nuclear Mitotic Apparatus Protein 1 antibody . Nuclear Mitotic Apparatus Protein 1 expression in wild type (WT) and Nuclear Mitotic Apparatus Protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Nuclear Mitotic Apparatus Protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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