This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of H9c2 cells with Phospho-PLCB3 (S537) antibody . Nuclei were stained blue with DAPI; Phospho-PLCB3 (S537) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-PLCB3 (S537) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PLCB3 (S537) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using phospho-PLCB3 (S537) antibody . Phospho-PLCB3 (S537) expression in wild-type (WT) and PLCB3 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-PLCB3 (S537) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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