This gene encodes a member of a subclass of the HSP40/DnaJ protein family. Members of this family of proteins are associated with complex assembly, protein folding, and export. Two transcript variants encoding distinct isoforms have been identified for this gene.

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Flow cytometric analysis of DNAJC12 expression in HeLa cells using DNAJC12 antibody . Green, isotype control; red, DNAJC12.

Immunocytochemical staining of HeLa cells with DNAJC12 antibody . Nuclei were stained blue with DAPI; DNAJC12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using DNAJC12 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNAJC12 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . DNAJC12, DnaJ heat shock protein family (Hsp40) member C12.

Western blotting analysis using DNAJC12 antibody . DNAJC12 expression in wild type (WT) and DNAJC12 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNAJC12 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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