Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.

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Flow cytometric analysis of Pyruvate dehydrogenase kinase 1 expression in H9c2 cells using Pyruvate dehydrogenase kinase 1 antibody . Green, isotype control; red, Pyruvate dehydrogenase kinase 1.

Immunocytochemical staining of H9C2 cells with Pyruvate dehydrogenase kinase 1 antibody . Nuclei were stained blue with DAPI; Pyruvate dehydrogenase kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Pyruvate dehydrogenase kinase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Pyruvate dehydrogenase kinase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Pyruvate dehydrogenase kinsae 1 antibody . Pyruvate dehydrogenase kinsae 1 expression in wild type (WT) and Pyruvate dehydrogenase kinsae 1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Pyruvate dehydrogenase kinsae 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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