This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22.

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Validation of Peroxiredoxin 3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Peroxiredoxin 3 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Peroxiredoxin 3 expression in HeLa cells using Peroxiredoxin 3 antibody . Green, isotype control; red, Peroxiredoxin 3.

Immunocytochemical staining of Hela cells with Peroxiredoxin 3 antibody . Nuclei were stained blue with DAPI; Peroxiredoxin 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Peroxiredoxin 3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Peroxiredoxin 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Peroxiredoxin 3 antibody . Peroxiredoxin 3 expression in wild type (WT) and Peroxiredoxin 3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Peroxiredoxin 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of Hela cells using Peroxiredoxin 3 antibody , Top panel: wild-type (WT); Bottom panal: Peroxiredoxin 3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Peroxiredoxin 3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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