This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of RAB4A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RAB4A antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells with RAB4A antibody . Nuclei were stained blue with DAPI; RAB4A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using RAB4A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAB4A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using RAB4A antibody . RAB4A expression in wild type (WT) and RAB4A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with RAB4A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using RAB4A antibody , Top panel: wild-type (WT); Bottom panal: Adiponectin receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RAB4A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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