This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified.

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Immunocytochemical staining of Hela cells using PA2G4 antibody , Top panel: wild-type (WT); Bottom panal: PA2G4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PA2G4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of PA2G4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PA2G4 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of PA2G4 expression in HepG2 cells using PA2G4 antibody . Green, isotype control; red, PA2G4.

Immunocytochemical staining of HepG2 cells with PA2G4 antibody . Nuclei were stained blue with DAPI; PA2G4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using PA2G4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PA2G4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using PA2G4 antibody . PA2G4 expression in wild type (WT) and PA2G4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PA2G4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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