The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of mTOR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-mTOR (S2448) antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Phospho-mTOR (S2448) expression in HeLa cells using Phospho-mTOR (S2448) antibody . Green, isotype control; red, Phospho-mTOR (S2448).

Immunocytochemical staining of HeLa cells with Phospho-mTOR (S2448) antibody . Nuclei were stained blue with DAPI; Phospho-mTOR (S2448) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-mTOR (S2448) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-mTOR (S2448) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phospho-mTOR (S2448) antibody . Phospho-mTOR (S2448) expression in wild type (WT) and Phospho-mTOR (S2448) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-mTOR (S2448) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Phospho-mTOR (S2448) antibody , Top panel: wild-type (WT); Bottom panal: Phospho-mTOR (S2448) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-mTOR (S2448) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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