The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of C2C12 cells with Phospho-MEK1 (S298) antibody . Nuclei were stained blue with DAPI; Phospho-MEK1 (S298) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-MEK1 (S298) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-MEK1 (S298) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phospho-MEK1 (S298) antibody . Phospho-MEK1 (S298) expression in wild type (WT) and Phospho-MEK1 (S298) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-MEK1 (S298) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of MEK1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-MEK1 (S298) antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Phospho-MEK1 (S298) antibody , Top panel: wild-type (WT); Bottom panal: Phospho-MEK1 (S298) shRNA knockdown (KD). Nuclei were stained blue with DAPI;Phospho-MEK1 (S298) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of Phospho-MEK1 (S298) expression in C2C12 cells using Phospho-MEK1 (S298) antibody . Green, isotype control; red, Phospho-MEK1 (S298).

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