Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Moesin antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Moesin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Moesin antibody . Moesin expression in wild type (WT) and Moesin shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Moesin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of Moesin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Moesin antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Moesin expression in HepG2 cells using Moesin antibody . Green, isotype control; red, Moesin.

Immunocytochemical staining of Hela cells with Moesin antibody . Nuclei were stained blue with DAPI; Moesin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunocytochemical staining of HeLa cells using Moesin antibody , Top panel: wild-type (WT); Bottom panal: Moesin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Moesin was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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