The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of NDUFB9 expression in C2C12 cells using NDUFB9 antibody . Green, isotype control; red, NDUFB9.

Immunocytochemical staining of C2C12 cells with NDUFB9 antibody . Nuclei were stained blue with DAPI; NDUFB9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using NDUFB9 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFB9 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using NDUFB9 antibody . NDUFB9 expression in wild type (WT) and NDUFB9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NDUFB9 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of NDUFB9 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NDUFB9 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using NDUFB9 antibody , Top panel: wild-type (WT); Bottom panal: NDUFB9 shRNA knockdown (KD). Nuclei were stained blue with DAPI;NDUFB9 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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