Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

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Validation of Mitochondrial ribosomal protein S15 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Mitochondrial ribosomal protein S15 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Mitochondrial ribosomal protein S15 expression in HepG2 cells using Mitochondrial ribosomal protein S15 antibody . Green, isotype control; red, Mitochondrial ribosomal protein S15.

Immunocytochemical staining of HepG2 cells with Mitochondrial ribosomal protein S15 antibody . Nuclei were stained blue with DAPI; Mitochondrial ribosomal protein S15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Mitochondrial ribosomal protein S15 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Mitochondrial ribosomal protein S15 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Mitochondrial ribosomal protein S15 antibody . Mitochondrial ribosomal protein S15 expression in wild type (WT) and Mitochondrial ribosomal protein S15 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Mitochondrial ribosomal protein S15 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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