This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.

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Western blotting analysis using Neuronal calcium sensor 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Neuronal calcium sensor 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Neuronal calcium sensor 1 antibody . Neuronal calcium sensor 1 expression in wild type (WT) and Neuronal calcium sensor 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Neuronal calcium sensor 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Flow cytometric analysis of Neuronal calcium sensor 1 expression in HepG2 cells using Neuronal calcium sensor 1 antibody . Green, isotype control; red, Neuronal calcium sensor 1.

Immunocytochemical staining of HepG2 cells with Neuronal calcium sensor 1 antibody . Nuclei were stained blue with DAPI; Neuronal calcium sensor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Validation of Neuronal calcium sensor 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Neuronal calcium sensor 1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Neuronal calcium sensor 1 antibody , Top panel: wild-type (WT); Bottom panal: Neuronal calcium sensor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Neuronal calcium sensor 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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