This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HeLa cells using eukaryotic translation elongation factor 2 antibody , Top panel: wild-type (WT); Bottom panal: eukaryotic translation elongation factor 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of Eukaryotic translation elongation factor 2 expression in HepG2 cells using Eukaryotic translation elongation factor 2 antibody . Green, isotype control; red, Eukaryotic translation elongation factor 2.

Immunocytochemical staining of HepG2 cells with Eukaryotic translation elongation factor 2 antibody . Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Eukaryotic translation elongation factor 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Eukaryotic translation elongation factor 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Eukaryotic translation elongation factor 2 antibody . Eukaryotic translation elongation factor 2 expression in wild type (WT) and Eukaryotic translation elongation factor 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Eukaryotic translation elongation factor 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Eukaryotic translation elongation factor 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Eukaryotic translation elongation factor 2 antibody and analyzed using BD flow cytometer.

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