The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms (PMID:12105216).

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of JunD expression in HepG2 cells using JunD antibody . Green, isotype control; red, JunD.

Immunocytochemical staining of HepG2 cells with JunD antibody . Nuclei were stained blue with DAPI; JunD was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using JunD antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with JunD antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using JunD antibody . JunD expression in wild type (WT) and JunD shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with JunD antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of JUND knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with JunD antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using JunD antibody , Top panel: wild-type (WT); Bottom panal: JUND shRNA knockdown (KD). Nuclei were stained blue with DAPI; JunD was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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