Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

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Immunocytochemical staining of HAP-1 cells with GST3 / GSTP1 antibody . Nuclei were stained blue with DAPI; GST3 / GSTP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of GST3 / GSTP1 expression in HAP-1 cells using GST3 / GSTP1 antibody (Cat #62189, 1:2,000). Green, isotype control; red, GST3 / GSTP1.

Western blotting analysis using GST3 / GSTP1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GST3 / GSTP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using GST3 / GSTP1 antibody . GST3 / GSTP1 expression in wild type (WT) and GST3 / GSTP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GST3 / GSTP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using GST3/GSTP1 antibody , Top panel: wild-type (WT); Bottom panal: GSTP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GST3/GSTP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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