Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009

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Flow cytometric analysis of Lysine demethylase 1B expression in HepG2 cells using Lysine demethylase 1B antibody . Green, isotype control; red, Lysine demethylase 1B.

Immunocytochemical staining of HepG2 cells with Lysine demethylase 1B antibody . Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Lysine demethylase 1B antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Lysine demethylase 1B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Lysine demethylase 1B antibody . Lysine demethylase 1B expression in wild type (WT) and Lysine demethylase 1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Lysine demethylase 1B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Lysine demethylase 1B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Lysine demethylase 1B antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Lysine demethylase 1B antibody , Top panel: wild-type (WT); Bottom panal: Lysine demethylase 1B shRNA knockdown (KD). Nuclei were stained blue with DAPI; Lysine demethylase 1B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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