This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

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Validation of GEMIN2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with GEMIN2 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of GEMIN2 expression in HepG2 cells using GEMIN2 antibody . Green, isotype control; red, GEMIN2.

Immunocytochemical staining of HepG2 cells with GEMIN2 antibody . Nuclei were stained blue with DAPI; GEMIN2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using GEMIN2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GEMIN2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using GEMIN2 antibody . GEMIN2 expression in wild type (WT) and GEMIN2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GEMIN2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of Hela cells using GEMIN2 antibody , Top panel: wild-type (WT); Bottom panal: GEMIN2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GEMIN2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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