This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.

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Flow cytometric analysis of Glucose-6-phosphate isomerase expression in HeLa cells using Glucose-6-phosphate isomerase antibody . Green, isotype control; red, Glucose-6-phosphate isomerase.

Immunocytochemical staining of Hela cells with Glucose-6-phosphate isomerase antibody (Cat #62019, 1:1,000) . Nuclei were stained blue with DAPI; Glucose-6-phosphate isomerase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Glucose-6-phosphate isomerase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glucose-6-phosphate isomerase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Glucose 6 phosphate isomerase antibody . Glucose 6 phosphate isomerase expression in wild type (WT) and Glucose 6 phosphate isomerase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Glucose 6 phosphate isomerase antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Glucose-6-phosphate isomerase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glucose-6-phosphate isomerase antibody and analyzed using BD flow cytometer.

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