Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Glutamic-oxaloacetic transaminase 1 expression in C2C12 cells using Glutamic-oxaloacetic transaminase 1 antibody . Green, isotype control; red, Glutamic-oxaloacetic transaminase 1.

Immunocytochemical staining of C2C12 cells with Glutamic-oxaloacetic transaminase 1 antibody (Cat #62017, 1:1,000) . Nuclei were stained blue with DAPI; Glutamic-oxaloacetic transaminase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using Glutamic-oxaloacetic transaminase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glutamic-oxaloacetic transaminase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Glutamic-oxaloacetic transaminase 1 antibody . Glutamic-oxaloacetic transaminase 1 expression in wild type (WT) and Glutamic-oxaloacetic transaminase 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Glutamic-oxaloacetic transaminase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Glutamic-oxaloacetic transaminase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glutamic-oxaloacetic transaminase 1 antibody and analyzed using BD flow cytometer.

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