The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Alternative splicing results in multiple transcript variants of this gene.

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Flow cytometric analysis of Golgi reassembly stacking protein 1 expression in HeLa cells using Golgi reassembly stacking protein 1 antibody . Green, isotype control; red, Golgi reassembly stacking protein 1.

Immunocytochemical staining of Hela cells with Golgi reassembly stacking protein 1 antibody (Cat #62016, 1:1,000). Nuclei were stained blue with DAPI; Golgi reassembly stacking protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Golgi reassembly stacking protein 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Golgi reassembly stacking protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Golgi reassembly stacking protein 1 antibody . Golgi reassembly stacking protein 1 expression in wild type (WT) and Golgi reassembly stacking protein 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Golgi reassembly stacking protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Golgi reassembly stacking protein 1 antibody , Top panel: wild-type (WT); Bottom panal: Golgi reassembly stacking protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Golgi reassembly stacking protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Validation of Golgi reassembly stacking protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Golgi reassembly stacking protein 1 antibody and analyzed using BD flow cytometer.

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