This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.

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Flow cytometric analysis of POLD1 expression in HepG2 cells using POLD1 antibody . Green, isotype control; red, POLD1.

Immunocytochemical staining of HAP-1 cells with POLD1 antibody . Nuclei were stained blue with DAPI; POLD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using POLD1 antibody . Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLD1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using POLD1 antibody . POLD1 expression in wild-type (WT) and POLD1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with POLD1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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