Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A rare single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described.

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Immunocytochemical staining of HeLa cells using Nudix hydrolase 1 antibody , Top panel: wild-type (WT); Bottom panal: Nudix hydrolase 1 shRNA knockdown (KD). Nuclei were stained blue with DAPINudix hydrolase 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Nudix hydrolase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Nudix hydrolase 1 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Nudix hydrolase 1 expression in HeLa cells using Nudix hydrolase 1 antibody . Green, isotype control; red, Nudix hydrolase 1.

Immunocytochemical staining of HeLa cells with Nudix hydrolase 1 antibody . Nuclei were stained blue with DAPI; Nudix hydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Nudix hydrolase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Nudix hydrolase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Nudix hydrolase 1 antibody . Nudix hydrolase 1 expression in wild type (WT) and Nudix hydrolase 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Nudix hydrolase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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