The protein encoded by this gene is a member of kinesin-like protein family. This family includes microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. This protein has been shown to cross-bridge antiparallel microtubules and drive microtubule movement in vitro. Alternate splicing of this gene results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HeLa cells using Kinesin family member 23 antibody , Top panel: wild-type (WT); Bottom panal: Kinesin family member 23 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Kinesin family member 23 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of Kinesin family member 23 expression in HepG2 cells using Kinesin family member 23 antibody . Green, isotype control; red, Kinesin family member 23.

Immunocytochemical staining of HepG2 cells with Kinesin family member 23 antibody . Nuclei were stained blue with DAPI; Kinesin family member 23 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Kinesin family member 23 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Kinesin family member 23 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Kinesin family member 23 antibody . Kinesin family member 23 expression in wild type (WT) and Kinesin family member 23 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Kinesin family member 23 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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