Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein.

推荐稀释比 WB: 1/2000-1/10000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of TSPO expression in HT-1080 cells using TSPO antibody . Green, isotype control; red, TSPO.

Immunocytochemical staining of HT-1080 cells with TSPO antibody . Nuclei were stained blue with DAPI; TSPO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using TSPO antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TSPO antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using TSPO antibody . TSPO expression in wild type (WT) and TSPO knockdown (KD) HSHC cells with 10 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TSPO antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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