This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Amyloid beta precursor like protein 2 expression in HepG2 cells using Amyloid beta precursor like protein 2 antibody . Green, isotype control; red, Amyloid beta precursor like protein 2.

Immunocytochemical staining of HepG2 cells with Amyloid beta precursor like protein 2 antibody . Nuclei were stained blue with DAPI; Amyloid beta precursor like protein 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Amyloid beta precursor like protein 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Amyloid beta precursor like protein 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using amyloid beta precursor like protein 2 antibody . Amyloid beta precursor like protein 2 expression in wild type (WT) and amyloid beta precursor like protein 2 (APLP2) shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with amyloid beta precursor like protein 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Amyloid beta precursor like protein 2 antibody , Top panel: wild-type (WT); Bottom panal: Amyloid beta precursor like protein 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Amyloid beta precursor like protein 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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