The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Atg16L1 expression in HT-1080 cells using Atg16L1 antibody . Green, isotype control; red, Atg16L1.

Immunocytochemical staining of HT-1080 cells with Atg16L1 antibody . Nuclei were stained blue with DAPI; Atg16L1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Atg16L1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Atg16L1 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Atg16L1 antibody . Atg16L1 expression in wild type (WT) and Atg16L1 shRNA knockdown (KD) 293T cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Atg16L1 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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