Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Rho GTPase activating protein 5 expression in C2C12 cells using Rho GTPase activating protein 5 antibody . Green, isotype control; red, Rho GTPase activating protein 5.

Immunocytochemical staining of C2C12 cells with Rho GTPase activating protein 5 antibody . Nuclei were stained blue with DAPI; Rho GTPase activating protein 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Rho GTPase activating protein 5 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Rho GTPase activating protein 5 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Rho GTPase activating protein 5 antibody . Rho GTPase activating protein 5 expression in wild type (WT) and Rho GTPase activating protein 5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Rho GTPase activating protein 5 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Rho GTPase activating protein 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Rho GTPase activating protein 5 antibody and analyzed using BD flow cytometer.

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