The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein.

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Western blotting analysis using C1QBP antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with C1QBP antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using C1QBP antibody . C1QBP expression in wild type (WT) and C1QBP shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with C1QBP antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using C1QBP antibody . C1QBP expression in wild type (WT) and C1QBP knockout (KO) 293T cells with 30 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with C1QBP antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of C1QBP expression in HepG2 cells using C1QBP antibody . Green, isotype control; red, C1QBP.

Immunocytochemical staining of HepG2 cells with C1QBP antibody . Nuclei were stained blue with DAPI; C1QBP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Validation of C1QBP knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with C1QBP antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using C1QBP antibody , Top panel: wild-type (WT); Bottom panal: C1QBP shRNA knockdown (KD). Nuclei were stained blue with DAPI; C1QBP was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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