This gene encodes a member of the bromodomain protein family. The bromodomain is a structural motif characteristic of proteins involved in chromatin-dependent regulation of transcription. This gene is deleted in Williams-Beuren syndrome, a developmental disorder caused by deletion of multiple genes at 7q11.23.

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Flow cytometric analysis of BAZ1B expression in HeLa cells using BAZ1B antibody . Green, isotype control; red, BAZ1B.

Immunocytochemical staining of Hela cells with BAZ1B antibody . Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using BAZ1B antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BAZ1B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using BAZ1B antibody . BAZ1B expression in wild type (WT) and BAZ1B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BAZ1B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using BAZ1B antibody , Top panel: wild-type (WT); Bottom panal: BAZ1B shRNA knockdown (KD). Nuclei were stained blue with DAPI; BAZ1B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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