This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.

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Immunocytochemical staining of HepG2 cells with Nuclear factor kappa B subunit 2 antibody . Nuclei were stained blue with DAPI; Nuclear factor kappa B subunit 2 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of Nuclear factor kappa B subunit 2 expression in HepG2 cells using Nuclear factor kappa B subunit 2 antibody . Green, isotype control; red, Nuclear factor kappa B subunit 2.

Western blotting analysis using nuclear factor kappa B subunit 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with nuclear factor kappa B subunit 2 antibody and HRP-conjugated goat anti rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using nuclear factor kappa B subunit 2 antibody . Nuclear factor kappa B subunit 2 expression in wild-type (WT) and nuclear factor kappa B subunit 2 (NFKB2) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with nuclear factor kappa B subunit 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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