A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

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Validation of Chondroitin sulfate proteoglycan 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Chondroitin sulfate proteoglycan 4 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Chondroitin sulfate proteoglycan 4 expression in C2C12 cells using Chondroitin sulfate proteoglycan 4 antibody . Green, isotype control; red, Chondroitin sulfate proteoglycan 4.

Immunocytochemical staining of C2C12 cells with Chondroitin sulfate proteoglycan 4 antibody . Nuclei were stained blue with DAPI; Chondroitin sulfate proteoglycan 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Chondroitin sulfate proteoglycan 4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Chondroitin sulfate proteoglycan 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Chondroitin sulfate proteoglycan 4 antibody . Chondroitin sulfate proteoglycan 4 expression in wild type (WT) and Chondroitin sulfate proteoglycan 4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Chondroitin sulfate proteoglycan 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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